co staining  (Proteintech)

Journal: bioRxiv

Article Title: Genotype-phenotype correlations in Wilms tumor initiation

doi: 10.1101/2025.11.19.689177

Figure Lengend Snippet: (A) Schematic representation of the Foxd1 GC lineage specific knockout of Wt1 in the mouse model. (B) Ex vivo kidney organ culture of E12.5 control (left) and Wt1 mutant kidney (right), with eGFP linked to FOXD1 expression and TdTomato representing the lineage. Scale 200µm. (C) Absolute quantification of the number of cap mesenchymes in the control versus mutant kidney, quantified from the ex vivo kidney organ culture. (D) Staining of DAPI and CDH1 on the ex vivo kidney culture. Scale 100µm. (E) H&E staining of control and Wt1 mutant kidneys at E12.5. Scale 50µm. (F) Staining for TdTomato to validate lineage and WT1 to validate knockout in E12.5 control and mutant kidneys. Scale 100µm. White arrowheads indicate Foxd1 lineage contribution to SIX2+ cells. (G) H&E staining of control and Wt1 mutant kidneys at E18.5. Scale 50µm. (H) Staining for TdTomato to validate lineage and WT1 to validate knockout in E18.5 control and mutant kidneys. Scale 100µm. White open arrows indicate Foxd1-lineage in or around proximal tubules.

Article Snippet: Secondary antibodies were incubated o/n at 4°C, followed by co-staining for DAPI (Polysciences, 09224-10) and mounting with FluorSave reagent (Merck Millipore, 345789).

Techniques: Knock-Out, Ex Vivo, Organ Culture, Control, Mutagenesis, Expressing, Quantitative Proteomics, Staining

Journal: British Journal of Cancer

Article Title: CD44 upregulation in chronic liver disease marks the transition to hepatocellular carcinoma and portends poor prognosis

doi: 10.1038/s41416-025-03284-y

Figure Lengend Snippet: a Representative images of CD44 IHC in injury model livers (arrows indicate CD44-positive hepatocytes). b CD44v6 IHC in injury models (arrows indicate CD44v6-positive hepatocytes or cholangiocytes). c Quantification of CD44 and ( d ) CD44v6 expression based on integrated optical density (IOD) in the indicated liver injury models. e Representative images of liver sections from CCl 4 -treated mice at 4 weeks and 16 weeks, as well as an HCC. H&E staining, Sirius Red staining (fibrosis in red), and CD44 IHC (red arrows point to CD44-positive hepatocytes) are shown. f Quantification of CD44-positive hepatocytes in CCl 4 -treated mice over time. *= p < 0.05; **= p < 0.01.

Article Snippet: After blocking with 10% normal goat or rabbit serum containing 3% BSA in TBST, sections were incubated overnight at 4 °C with three sets of primary antibodies for co-staining: CD44 (#37259, Cell Signaling, 1:200) together with FOXP3 (#14-4771-80, ThermoFisher, 1:200), CD44 (IM7, #14-0441-82, ThermoFisher, 1:400) together with F4/80 (#70076, Cell Signaling, 1:200) and CD44 (#37259, Cell Signaling, 1:200) together with CD206 (#PA5-46994, ThermoFisher, 1:200).

Techniques: Expressing, Staining

Journal: British Journal of Cancer

Article Title: CD44 upregulation in chronic liver disease marks the transition to hepatocellular carcinoma and portends poor prognosis

doi: 10.1038/s41416-025-03284-y

Figure Lengend Snippet: a CD44 mRNA expression (TPM) in TCGA HCC tumours vs. adjacent normal liver (NTL). b CD44 mRNA levels by tumour stage (TCGA HCC). c Representative immunohistochemistry images of normal liver, adjacent non-tumorous liver, HCC, and iCCA tissues (images retrieved from the Human Protein Atlas). d Kaplan–Meier overall survival curves for TCGA HCC patients with CD44 high vs. CD44 low tumours. e Validation of survival difference in CD44 high vs. CD44 low groups using GEPIA2 (combined TCGA/GTEx data). ****= p < 0.0001.

Article Snippet: After blocking with 10% normal goat or rabbit serum containing 3% BSA in TBST, sections were incubated overnight at 4 °C with three sets of primary antibodies for co-staining: CD44 (#37259, Cell Signaling, 1:200) together with FOXP3 (#14-4771-80, ThermoFisher, 1:200), CD44 (IM7, #14-0441-82, ThermoFisher, 1:400) together with F4/80 (#70076, Cell Signaling, 1:200) and CD44 (#37259, Cell Signaling, 1:200) together with CD206 (#PA5-46994, ThermoFisher, 1:200).

Techniques: Expressing, Immunohistochemistry, Biomarker Discovery

Journal: British Journal of Cancer

Article Title: CD44 upregulation in chronic liver disease marks the transition to hepatocellular carcinoma and portends poor prognosis

doi: 10.1038/s41416-025-03284-y

Figure Lengend Snippet: a Volcano plot of differentially expressed genes in CD44 high vs. CD44 low HCC (red dots = upregulated genes; green dots = downregulated genes; selected genes labelled). b Top KEGG pathways enriched in CD44 high tumours (bar graph of -log 10 P values). c GSVA enrichment scores for selected oncogenic pathways (IL6/JAK/STAT3, TNFα/NF-κB, EMT, etc.) in CD44 high vs. CD44 low tumours. d Immune cell fraction comparison (xCell analysis) showing higher M2 macrophage and Th2 cell signatures in CD44 high tumours. *= p < 0.05; **= p < 0.01; ***= p < 0.001, ****= p < 0.0001.

Article Snippet: After blocking with 10% normal goat or rabbit serum containing 3% BSA in TBST, sections were incubated overnight at 4 °C with three sets of primary antibodies for co-staining: CD44 (#37259, Cell Signaling, 1:200) together with FOXP3 (#14-4771-80, ThermoFisher, 1:200), CD44 (IM7, #14-0441-82, ThermoFisher, 1:400) together with F4/80 (#70076, Cell Signaling, 1:200) and CD44 (#37259, Cell Signaling, 1:200) together with CD206 (#PA5-46994, ThermoFisher, 1:200).

Techniques: Comparison

Journal: British Journal of Cancer

Article Title: CD44 upregulation in chronic liver disease marks the transition to hepatocellular carcinoma and portends poor prognosis

doi: 10.1038/s41416-025-03284-y

Figure Lengend Snippet: UMAP plots of HCC ( a ) and iCCA ( d ) tumours derived from the single-cell RNA-seq datasets GSE166635 (HCC) and GSE138709 (iCCA), with major cell populations annotated (macrophages, T cells, and malignant cells). Feature plots showing CD44 expression levels across specific cell populations in HCC ( b ) and iCCA ( e ) samples (blue = high expression, grey = low expression). UMAP clustering of normal tissues (blue) versus tumour cells (orange) in an HCC sample ( c ) and an iCCA sample ( f ).

Article Snippet: After blocking with 10% normal goat or rabbit serum containing 3% BSA in TBST, sections were incubated overnight at 4 °C with three sets of primary antibodies for co-staining: CD44 (#37259, Cell Signaling, 1:200) together with FOXP3 (#14-4771-80, ThermoFisher, 1:200), CD44 (IM7, #14-0441-82, ThermoFisher, 1:400) together with F4/80 (#70076, Cell Signaling, 1:200) and CD44 (#37259, Cell Signaling, 1:200) together with CD206 (#PA5-46994, ThermoFisher, 1:200).

Techniques: Derivative Assay, RNA Sequencing, Expressing